CIZ1 Polyclonal antibody

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  WB analysis using 31683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31683-1-AP (CIZ1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CIZ1 Polyclonal Antibody for WB, ELISA

Cat No. 31683-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZNF356, NP94, LSFR1, CIZ 1, Cip1-interacting zinc finger protein

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31683-1-AP targets CIZ1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIZ1 fusion protein Ag35418
• Full Name: CDKN1A interacting zinc finger protein 1
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC021163
• Gene Symbol: CIZ1
• Gene ID (NCBI): 25792
• RRID: AB_3670077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9ULV3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIZ1, also known as LSFR1 and ZNF356, is a nuclear protein and the binding partner of p21. The full-length CIZ1 consists of 842 amino acid (aa) residues and contains two glutamine-rich domains, three C2H2-type zinc finger motifs, one acidic domain, and one MH3 domain (PMID: 10529385). Dysregulated or point mutated CIZ1 has been implicated in neurodegenerative, autoimmune and cancer diseases.