CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17760-1-AP

Synonyms

CLABP, Clipin A, CLIPINA, CORO1, CORO1A, Coronin 1A, Coronin like protein A, Coronin like protein p57, HCORO1, p57, TACO



Tested Applications

Positive WB detected inrat brain tissue, Jurkat cells, HL-60 cells, rat spleen tissue, mouse spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inrat spleen tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue, mouse colon tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17760-1-AP targets CORO1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO1A fusion protein Ag12139
Full Name coronin, actin binding protein, 1A
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC110374
Gene Symbol CORO1A
Gene ID (NCBI) 11151
RRIDAB_2082070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CORO1A antibody 17760-1-APDownload protocol
IHC protocol for CORO1A antibody 17760-1-APDownload protocol
IF protocol for CORO1A antibody 17760-1-APDownload protocol
IP protocol for CORO1A antibody 17760-1-APDownload protocol
FC protocol for CORO1A antibody 17760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Br J Pharmacol

PMID: 27128203

Authors - Xianrong Zhang
humanWB

Int Immunopharmacol

Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash.

Authors - Fuli Min
humanWB

Front Med (Lausanne)

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Authors - Hui Song
ratWB

PLoS One

Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia.

Authors - Feng Guo