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  • KD/KO Validated

CORO1C Polyclonal antibody

CORO1C Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14749-1-AP

Synonyms

CORO1C, Coronin 1C, Coronin 3, CRNN4, HCRNN4



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, SGC-7901 cells, HeLa cells, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14749-1-AP targets CORO1C in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO1C fusion protein Ag6488
Full Name coronin, actin binding protein, 1C
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC002342
Gene Symbol CORO1C
Gene ID (NCBI) 23603
RRIDAB_2230110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CORO1C antibody 14749-1-APDownload protocol
IHC protocol for CORO1C antibody 14749-1-APDownload protocol
IF protocol for CORO1C antibody 14749-1-APDownload protocol
IP protocol for CORO1C antibody 14749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyWB

Cell

A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission.

Authors - Melissa J Hoyer
  • KD Validated
humanWB

J Cell Biol

Coronin 1C restricts endosomal branched actin to organize ER contact and endosome fission.

Authors - Jonathan F Striepen
mouseWB

Hum Mol Genet

Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice.

Authors - Laura Pellegrini
humanWB, IHC

Front Mol Biosci

CORO1C is Associated With Poor Prognosis and Promotes Metastasis Through PI3K/AKT Pathway in Colorectal Cancer.

Authors - Zongxia Wang
humanWB

Genes (Basel)

Coronin 1C, Regulated by Multiple microRNAs, Facilitates Cancer Cell Aggressiveness in Pancreatic Ductal Adenocarcinoma

Authors - Kosuke Fukuda
humanIHC, WB

Oncol Rep

microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma.

Authors - Jianhong Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wen (Verified Customer) (12-22-2019)

Work well in western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse haematopoietic cells