Filter: AllWBIHCIF/ICC

WB analysis using 55195-1-AP (same clone as 55195-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 55195-1-AP (same clone as 55195-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 55195-1-AP (same clone as 55195-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 55195-1-AP (same clone as 55195-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 55195-1-AP (same clone as 55195-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 55195-1-AP (same clone as 55195-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 55195-1-AP (same clone as 55195-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 55195-1-AP (same clone as 55195-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 55195-1-AP (same clone as 55195-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 55195-1-AP (same clone as 55195-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 55195-1-AP (same clone as 55195-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 55195-1-AP (same clone as 55195-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (55195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

CPSF7 Polyclonal antibody, PBS Only

CPSF7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55195-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

55195-1-PBS targets CPSF7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	pre-mRNA cleavage factor I, 59 kDa subunit
Calculated Molecular Weight	56 kDa
Observed Molecular Weight	52 kDa, 56 kDa
GenBank Accession Number	NM_001136040
Gene Symbol	CPSF7
Gene ID (NCBI)	79869
RRID	AB_10858797
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N684
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.