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Cathepsin B Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 12216-1-AP

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Con: 49 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

Cathepsin B Antibody WB A375 cells 12216-1-AP

A375 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Cathepsin B Antibody IHC human ovary tumor tissue 12216-1-AP

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP( Cathepsin B Antibody) at dilution of 1:200 (under 10x lens).

Cathepsin B Antibody IHC human ovary tumor tissue 12216-1-AP

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP( Cathepsin B Antibody) at dilution of 1:200 (under 40x lens).

Cathepsin B Antibody IF A375 cells 12216-1-AP

Immunofluorescent analysis of A375 cells using 12216-1-AP(Cathepsin B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Cathepsin B Antibody FC MCF-7 cells 12216-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug Cathepsin B antibody (12216-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, frog, rat
Positive WB detected in:
A375 cells, HT-1080 cells, human brain tissue, human liver tissue, mouse brain tissue, NIH/3T3 cells
Positive IHC detected in:
human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A375 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:100-1:400
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cathepsin B fusion protein Ag2898
Full name:
cathepsin B
Calculated molecular weight:
339aa,38 kDa
Observed molecular weight:
38 kDa, 44 kDa
GenBank accession number:
BC010240
Gene ID (NCBI):
1508
Gene symbol
CTSB
Synonyms
APP secretase, APPS, cathepsin B, Cathepsin B1, CPSB, CTSB

Background

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa marure forms in mouse brain by western blot(PMID:20616152).


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