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CoraLite® Plus 647-conjugated DNAJB1 Polyclonal antibody

DNAJB1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13174

Synonyms

DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13174 targets DNAJB1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated Molecular Weight 340 aa, 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC019827
Gene Symbol DNAJB1
Gene ID (NCBI) 3337
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 DNAJB1 antibody CL647-13174Download protocol
FC protocol for CL Plus 647 DNAJB1 antibody CL647-13174Download protocol
Standard Protocols
Click here to view our Standard Protocols