Product Information
FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 is a novel antibody labeling kit that uses an affinity linker to conjugate CoraLite® Plus 555 in any buffer condition to mouse IgG1 primary antibodies from any supplier. One labeling reaction requires only 0.5µg of antibody – regardless of the antibody concentration. Label your mouse IgG1 antibody in<10 min without any additional equipment.
| Product name | FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 |
| Assay type | Antibody labeling |
| Tested applications | IF, FC, WB |
| Species Reactivity | Mouse IgG1 |
| Antibody amount per labeling reaction | 0.5 µg antibody |
| Conjugate | CoraLite® Plus 555 |
| Excitation/Emission maxima wavelengths | 554 nm / 570 nm |
Kit Components
| Component | 10 rxns | 50 rxns | 200rxns |
| FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 | 50rxns | ||
| FlexAble CoraLite®Plus 550 FlexLinker for mouse IgG1 | 10ul | 50ul | |
| FlexBuffer | 100ul | 500ul | |
| FlexQuencher for Mouse IgG1 | 20ul | 100ul |
Storage Condition
Store for 1 year at -20°C or for 6 months at +4°C upon receipt. Avoid exposure to lightFAQ
Q: What are the FlexLinker, FlexQuencher and FlexBuffer?
A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.
Q: What is the largest quantity I can label?
A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.
Q: What is the lowest concentration of my primary antibody that I can use?
A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.
Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?
A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.
Q: How many different primary antibodies can I label with one kit?
A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.
Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?
A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies.
Publications
| Application | Title |
|---|---|
J Cell Sci Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types | |
Nat Commun UPP1 promotes lung adenocarcinoma progression through the induction of an immunosuppressive microenvironment |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alessandro (Verified Customer) (12-09-2023) | New FlexAble kits guarantee precise immunolabeling of a primary antibody cutting waiting times in half
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