Human CXCL16 ELISA Kit

Sensitivity

0.1 pg/mL

Range

7.8-500 pg/mL

Reactivity

Human

Cat no : KE00052

Synonyms

C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16



Product Information

KE00052 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL16 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL16. The assay recognizes human CXCL16. An antibody specific for CXCL16 has been pre-coated onto the microwells. The CXCL16 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CXCL16 is added to detect the captured CXCL16 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human CXCL16 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.1 pg/mL
Range 7.8-500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 58191

Recovery

Sample TypeAverageRange
Human serum91%76%-103%
Human plasma99%83%-119%
Cell culture supernatants87%82%-118%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120241.110.84.5
22056.11.73.0
32012.70.53.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124260.717.16.6
22456.03.25.8
32412.70.75.8

Background Information

CXC chemokine ligand 16 (CXCL16) is a CXC soluble chemokine, an adhesion molecule and a cell surface scavenger receptor. CXCL16 regulates inflammation, tissue injury and fibrosis. CXCL16 exists as a transmembrane form (TM-CXCL16) as well as a soluble form (sCXCL16) that is cleaved by proteolytic enzymes. CXCL16 is produced by several inflammatory cells including macrophages, dendritic cells, smooth muscle cells or lymphocytes. CXCL16 is expressed in various cancers, including pancreatic, prostate, breast, colorectal, and nonsmall cell lung cancer. CXCL16) has been proposed as an important pathogenic mediator in inflammatory diseases, such as rheumatoid arthritis, hepatitis or atherosclerosis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Am J Pathol

Low-Dose Acetylsalicylic Acid Treatment Modulates the Production of Cytokines and Improves Trophoblast Function in an in Vitro Model of Early-Onset Preeclampsia.

Authors - Shanika Panagodage
humanserum

Exp Dermatol

Reduced expression of CXCL16/CXCR6 is involved in the pathogenesis of hidradenitis suppurativa

Authors - Zhongshuai Wang