Speedy™ Human Tetranectin One-Step ELISA Kit
Cat no : SE50207
Synonyms
CLEC3B, C-type lectin domain family 3 member B, Plasminogen kringle 4-binding protein, TN, TNA
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Tetranectin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 22.2 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7123 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 96% | 90%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,486.2 | 84.6 | 3.4 |
| 2 | 8 | 666.3 | 60.5 | 9.1 |
| 3 | 8 | 344.8 | 32.0 | 9.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,548.4 | 197.2 | 7.7 |
| 2 | 16 | 674.7 | 53.3 | 7.9 |
| 3 | 16 | 344.1 | 34.1 | 9.9 |
Background Information
Tetranectin (TN), which is encoded by the Clec3b gene, is a homotrimeric molecule of the C-type superfamily of lectins. TN was initially identified as a plasminogen-binding protein from human plasma, which shares an 87% amino acid sequence identity to its counterpart in mice. TN mRNA is detected in various tissues, but its protein expression is more tissue restricted. Several functions of TN have been reported including regulation of fibrinolysis and proteolytic processes, osteogenesis, myogenesis, Parkinson disease, and bone mineralization.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLEC3B, C-type lectin domain family 3 member B, Plasminogen kringle 4-binding protein, TN, TNA |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
