• Featured Product
  • KD/KO Validated

KCNN4 Polyclonal antibody

KCNN4 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 23271-1-AP

Synonyms

KCa3.1, KCNN4, SK4



Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HEK-293 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23271-1-AP is KCNN4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNN4 fusion protein Ag19774
Full Name potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
Calculated Molecular Weight 427 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC015337
Gene Symbol KCNN4
Gene ID (NCBI) 3783
RRIDAB_2879243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).

Protocols

Product Specific Protocols
WB protocol for KCNN4 antibody 23271-1-APDownload protocol
IF protocol for KCNN4 antibody 23271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancer Cell Int

KCNN4 may weaken anti-tumor immune response via raising Tregs and diminishing resting mast cells in clear cell renal cell carcinoma.

Authors - Yankang Cui
humanWB,IHC,FC

Cancer Biomark

KCNN4 promotes the progression of lung adenocarcinoma by activating the AKT and ERK signaling pathways

Authors - Ping Xu
  • KD Validated
humanWB

Front Physiol

New KCNN4 Variants Associated With Anemia: Stomatocytosis Without Erythrocyte Dehydration

Authors - B. Allegrini
humanIHC

Biochem Biophys Res Commun

Integrated expression profiling of potassium channels identifys KCNN4 as a prognostic biomarker of pancreatic cancer.

Authors - Shuheng Jiang
mouseWB

Gels

Comprehensive Optimization of Western Blotting

Authors - Dishiwen Liu
humanWB

Int J Mol Sci

Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages

Authors - Susumu Ohya