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L-Plastin Polyclonal antibody

L-Plastin Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 13025-1-AP

Synonyms

L PLASTIN, LCP 1, LCP1, L-Plastin, Lymphocyte cytosolic protein 1, Plastin 2, PLS2



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, mouse spleen tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13025-1-AP targets L-Plastin in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, hamster, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L-Plastin fusion protein Ag3571
Full Name lymphocyte cytosolic protein 1 (L-plastin)
Calculated Molecular Weight 627 aa, 64 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberBC010271
Gene Symbol LCP1
Gene ID (NCBI) 3936
RRIDAB_2136871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for L-Plastin antibody 13025-1-APDownload protocol
IHC protocol for L-Plastin antibody 13025-1-APDownload protocol
IP protocol for L-Plastin antibody 13025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Signal

A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity

Authors - Hyunbae Kim
humanWB,IHC

Mol Ther Nucleic Acids

Exosomal Transfer of LCP1 Promotes Osteosarcoma Cell Tumorigenesis and Metastasis by Activating the JAK2/STAT3 Signaling Pathway.

Authors - Xuhui Ge
humanIHC

Cancer Biomark

LCP1 is a prognostic biomarker correlated with immune infiltrates in gastric cancer

Authors - Qingwen Zeng
mouseWB

Drug Dev Res

Therapeutic role of Artemether in the prevention of hepatic steatosis through miR-34a-5p/PPARα pathway

Authors - Li Chen
monkeyIHC

Mol Biosyst

Unravelling the proteome of adult rhesus monkey ovaries.

Authors - Hui He
humanWB,FC

Pharm Res

Transcriptome Profiling Analysis Identifies LCP1 as a Contributor for Chidamide Resistance in Gastric Cancer.

Authors - Wenfang Bao
  • KD Validated