- Featured Product
- KD/KO Validated
CoraLite® Plus 647-conjugated MUM1/IRF4 Polyclonal antibody
MUM1/IRF4 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-11247
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-11247 targets MUM1/IRF4 in FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MUM1/IRF4 fusion protein Ag1762 |
Full Name | IRF 4 |
Calculated Molecular Weight | 52 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC015752 |
Gene Symbol | IRF4 |
Gene ID (NCBI) | 3662 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
Protocols
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 647 MUM1/IRF4 antibody CL647-11247 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |