For Research Use Only
 
Broad Range Prestained Protein Marker (3-245 kDa)
Catalog Number:PL00002


www.ptglab.com
Description
The PL00002 Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights from 3 to 245 kDa in Tris-Glycine buffer. The PL00002 Broad Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins.
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage
Store product at 4 C for up to 12 weeks. For longer storage, aliquot and store at -20 C for up to 1 year.

*This product may ship at ambient temperature. Upon receiving this product, immediately store product at the recommended temperatures.

Instructions
The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.
Detection Method
Colorimetric
Molecular Weight
~ 3, 10, 15, 20, 25, 35, 45, 60, 75, 100, 140, 180, 245 kDa
Number of Markers
13
Product Size
100uL or 500uL
Size Range
3 to 245
Stain Type
3 colors: Blue, Red, Green
For technical support and original validation data for this product please contact:
T: 1 (888) 4PTGLAB
(1-888-478-4522) (toll free in USA),
or 1(312) 455-8498 (outside USA)
E: proteintech@ptglab.com

W: ptglab.com
This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
For Research Use Only
 
Broad Range Prestained Protein Marker (3-245 kDa)
Catalog Number:PL00002


www.ptglab.com

Figure 1. SDS-PAGE band profile of Broad Range Prestained Protein Ladder

Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

Figure 2 – Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions

Note on apparent molecular weights:  Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in Figure 2.

For technical support and original validation data for this product please contact:
T: 1 (888) 4PTGLAB
(1-888-478-4522) (toll free in USA),
or 1(312) 455-8498 (outside USA)
E: proteintech@ptglab.com

W: ptglab.com
This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.