PLA2G12B Polyclonal antibody

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  WB analysis using 25799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25799-1-AP (PLA2G12B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25799-1-AP

  WB result of PLA2G12B antibody (25799-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLA2G12B transfected HepG2 cells.

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  WB analysis of HuH-7 using 25799-1-AP

  WB result of PLA2G12B antibody (25799-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLA2G12B transfected HuH-7 cells.

• Featured Product
• KD/KO Validated

PLA2G12B Polyclonal Antibody for WB, ELISA

Cat No. 25799-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FKSG71, Group XIIB secretory phospholipase A2-like protein, Group XIII secretory phospholipase A2-like protein, GXIIB, GXIII sPLA2 like

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, HuH-7 cells, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25799-1-AP targets PLA2G12B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLA2G12B fusion protein Ag22877
• Full Name: phospholipase A2, group XIIB
• Calculated Molecular Weight: 195 aa, 22 kDa
• Observed Molecular Weight: 21-22 kDa
• GenBank Accession Number: BC111946
• Gene Symbol: PLA2G12B
• Gene ID (NCBI): 84647
• RRID: AB_3085823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BX93
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase A2 (PLA2) enzymes catalyze hydrolysis of glycolipids to release free fatty acids and lysophospholipids. PLA2G12B is a secreted group XIIB secretory phospholipase A2-like protein belonging to the PLA2 family, but it is catalytically inactive due to an amino acid change in its active site and has altered phospholipid-binding properties (PMID: 14516201). It is located outside the cell membranes and strongly expressed in liver, small intestine and kidney, and recently reports revealed that Pla2g12b gene may be implicated in HDL cholesterol levels and metabolism (PMID: 22912808). The calculated molecular weight of PLA2G12B is 21 kDa.