PPIL4 Polyclonal antibody

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  WB analysis using 12538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12538-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12538-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12538-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12538-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12538-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12538-1-AP

  IP result of anti-PPIL4 (IP:12538-1-AP, 4ug; Detection:12538-1-AP 1:300) with HeLa cells lysate 2600 ug.

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  IF Staining of HeLa using 12538-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPIL4 antibody (12538-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PPIL4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 12538-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Cyclophilin like protein PPIL4, Cyclophilin-like protein PPIL4, EC:5.2.1.8, HDCME13P, Peptidyl-prolyl cis-trans isomerase-like 4

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Tested Applications

• Positive WB detected in: HEK-293 cells, HCT 116 cells, mouse kidney tissue, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12538-1-AP targets PPIL4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPIL4 fusion protein Ag3249
• Full Name: peptidylprolyl isomerase (cyclophilin)-like 4
• Calculated Molecular Weight: 492 aa, 57 kDa
• Observed Molecular Weight: 58-65 kDa
• GenBank Accession Number: BC016984
• Gene Symbol: PPIL4
• Gene ID (NCBI): 85313
• RRID: AB_10644441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WUA2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peptidyl-prolyl isomerase (PPIase) catalyzes the interconversion of a specific Pro-imide bond between the cis and trans conformations. PPIL4 belongs to the cyclophilin subgroup of the peptidyl-prolyl cis-trans isomerase (PPIase) protein family (PPIs). It contains an RNA recognition motif and a PPIase-like domain. PPIL4, as an IA susceptibility gene, is a novel and key regulatory molecule in Wnt signaling and vertebrate cerebrovascular development (PMID: 34887573).

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction.; Authors - Wenbo Liu