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CoraLite® Plus 647-conjugated Phospho-GSK3B (Ser9) Monoclonal antibody

Phospho-GSK3B (Ser9) Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C9E2

Cat no : CL647-67558

Synonyms

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta, Phospho-GSK3B (Ser9)

Validation Data Gallery

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  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 647-conjugated Phospho-GSK3B (Ser9) (CL647-67558)

    FC experiment of PC-3 using CL647-67558

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.125 ug CoraLite® Plus 647 Anti-Human Phospho-GSK3B (Ser9) (CL647-67558, Clone:1C9E2) (red), or 0.125 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive FC detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67558 targets Phospho-GSK3B (Ser9) in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_002093
Gene Symbol GSK3B
Gene ID (NCBI) 2932
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .