CoraLite® Plus 647-conjugated RABEPK/p40 Polyclonal antibody

RABEPK/p40 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-15105

Synonyms

40 kDa Rab9 effector protein, bA65N13.1, DKFZp686P1077, p40, RAB9P40, RABEPK

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Validation Data Gallery

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  IF Staining of HeLa using CL647-15105

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 RABEPK/p40 antibody (CL647-15105) at dilution of 1:200.

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  FC experiment of HeLa using CL647-15105

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human RABEPK/p40 (CL647-15105) (red), or 0.2 ug CL647 rabbit Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-15105 targets RABEPK/p40 in IF, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RABEPK/p40 fusion protein Ag2593
• Full Name: Rab9 effector protein with kelch motifs
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC065725
• Gene Symbol: RABEPK
• Gene ID (NCBI): 10244
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.