SAMHD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SAMHD1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

65

Cat no : 12586-1-AP

Synonyms

DCIP, HDDC1, Monocyte protein 5, MOP 5, MOP5, SAM domain and HD domain 1, SAMHD1, SBBI88

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Validation Data Gallery

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  WB analysis using 12586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12586-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12586-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12586-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12586-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12586-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12586-1-AP

  IP result of anti-SAMHD1 (IP:12586-1-AP, 3ug; Detection:12586-1-AP 1:300) with K-562 cells lysate 2400ug.

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  IHC staining of human lung cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12586-1-AP (SAMHD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12586-1-AP (SAMHD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAMHD1 antibody (12586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of THP-1 using 12586-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.2 ug Anti-Human SAMHD1 (12586-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or 0.2 ug Control Antibody (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

• 

  FC experiment of K-562 using 12586-1-AP

  1X10^6 K-562 cells were stained with 0.2ug SAMHD1 antibody (12586-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, mouse heart tissue, human heart tissue, L02 cells, human brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: THP-1 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 14 publications below
• WB: See 42 publications below
• IHC: See 9 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• FC: See 11 publications below

Product Information

12586-1-AP targets SAMHD1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAMHD1 fusion protein Ag3287
• Full Name: SAM domain and HD domain 1
• Calculated Molecular Weight: 626 aa, 72 kDa
• Observed Molecular Weight: 64-72 kDa
• GenBank Accession Number: BC036450
• Gene Symbol: SAMHD1
• Gene ID (NCBI): 25939
• RRID: AB_2183496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.

Publications

Species	Application	Title
human	IF	Nat Genet; Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response.; Authors - Rice Gillian I GI
human	IF	Nat Microbiol; Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97.; Authors - Ramona Businger
human	WB,IHC	Nat Med; PMID: 27991919; Authors - Constanze Schneider
human	WB,IHC	Nat Med; PMID: 28067901; Authors - Nikolas Herold
human	WB, IHC, IF	Nat Med; SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells.; Authors - Baldauf Hanna-Mari HM ; KD Validated
monkey	WB	Immunity; Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells.; Authors - Nina Calantone

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