Siglec-H Polyclonal antibody

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  WB analysis using 33801-1-AP

  Mouse kidney tissue lysates were subjected to SDS PAGE followed by western blot with 33801-1-AP (Siglec-H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Siglec-H Polyclonal Antibody for WB, ELISA

Cat No. 33801-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Sialic acid binding Ig-like lectin H Siglec-H Siglech protein, Siglech

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Tested Applications

• Positive WB detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33801-1-AP targets Siglec-H in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg6216

  Product name: Recombinant mouse Siglec-H protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 19-263 aa of /

  Sequence: NCFILPREVIGTYPSLTLELQSVTVQEGLCVLVPCHLIEYPMNRWSDAAFGYWFHEGENTHPDSLVATNKPYQLVQKEMQSRFHFSGDCSLDIRDAQKGDNGSYFFRLEKGGKTWNFCEERISVHVTDLTHSPNISIPQTLELGRPTNVMCSVPWACERGTPPIFSWMSAALISLGPTTTLSSVLTLTPRLQDHGTNLTCQVTFPGAGVTVQKTIQLNVTYNSQNSTAHVSGRVGPGKPRPLADV

• Full Name: sialic acid binding Ig-like lectin H
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: /
• Gene Symbol: Siglech
• Gene ID (NCBI): 233274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q3Y597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sialic acid-binding immunoglobulin-like lectin H (Siglec-H) is a type I transmembrane receptor belonging to the Siglec family, characterized by its ability to recognize sialylated glycans. In mice, Siglec-H is selectively expressed on plasmacytoid dendritic cells (pDCs) and is widely used as a defining surface marker for this cell population. Unlike many inhibitory Siglecs, Siglec-H lacks classical ITIM motifs and instead associates with the adaptor protein DAP12 (TYROBP), enabling it to modulate intracellular signaling. Functionally, Siglec-H plays a role in regulating type I interferon production, endosomal Toll-like receptor (TLR7/9) responses, and antiviral immunity. It can act as a negative regulator of excessive interferon responses, thereby maintaining immune homeostasis. In mouse models, Siglec-H is implicated in viral infection, autoimmune diseases (e.g., lupus-like conditions), and immune tolerance. Due to its restricted expression and functional relevance, Siglec-H is a key target in pDC biology, innate immunity, and immunoregulation research.