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TDP-43 Polyclonal antibody

TDP-43 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (6)

Applications

WB, RIP, IP, IHC, IF, IEM, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10782-2-AP

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43



Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human brain (FTLD-U) tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:3000-1:12000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10782-2-AP targets TDP-43 in WB, RIP, IP, IHC, IF, IEM, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, chicken, horse, Yeast, seal, monkey, zebrafish, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC001487
Gene Symbol TARDBP
Gene ID (NCBI) 23435
RRIDAB_615042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

 The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Protocols

Product Specific Protocols
WB protocol for TDP-43 antibody 10782-2-APDownload protocol
IHC protocol for TDP-43 antibody 10782-2-APDownload protocol
IF protocol for TDP-43 antibody 10782-2-APDownload protocol
IP protocol for TDP-43 antibody 10782-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Lancet Neurol

A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study.

Authors - Ilse Gijselinck
mouseIF

Cell Res

Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies

Authors - Liang Guo
humanWB

Nature

MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.

Authors - Roberto Simone
humanIF

Nature

TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.

Authors - X Rosa Ma
mouseIF

Nature

Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.

Authors - Jiasheng Zhang
humanIP

Nature

TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.

Authors - Anna-Leigh Brown

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Paloma (Verified Customer) (03-26-2024)

We love this item since we start to use it.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:2000-1:8000
  • Cell Tissue Type: IHQ for neuroscience
FH

MANOHAR (Verified Customer) (03-06-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue
FH

Paloma (Verified Customer) (02-27-2024)

We have been using the TDP43 antibody for diagnosis of neurodegenerative diseases for a long time. We like this proteintech antibody very much, because its format and dilution makes our work much easier. In addition, the image it gives us after IHC is beautiful and helps us in the diagnosis.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:2000-1:8000
  • Cell Tissue Type: IHQ for neuroscience
FH

David (Verified Customer) (01-02-2024)

Top notch antibody, single band at predicted molecular weight. Gold standard.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y lysate
FH

Parijat (Verified Customer) (09-09-2023)

Good antibody for IF

  • Applications: Immunofluorescence
TDP-43 Antibody Immunofluorescence validation ( dilution) in (Cat no:10782-2-AP)
FH

Florencia (Verified Customer) (07-17-2023)

This antibody is one of the most important ones we use in the lab for studying ALS! It works very well and we will continue to use it.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: iPSC-derived neurons
FH

Pauline (Verified Customer) (02-23-2022)

everything ok

  • Applications: Immunofluorescence
  • Cell Tissue Type: HeLa
FH

Xin (Verified Customer) (01-23-2022)

Very good in WB with a band of around 45 kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain lysate
FH

Jessica (Verified Customer) (06-10-2021)

good for WB and IF

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
FH

Ana (Verified Customer) (03-14-2021)

TDP43 in human primary fibroblasts. 10 ug of total protein. Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST. Secondary ab Goat anti-rabbit HRP 1:5000 1h RT incubation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Primary human fibrosblasts
TDP-43 Antibody Western Blot validation (1:1000 dilution) in Primary human fibrosblasts (Cat no:10782-2-AP)
FH

Yasuyo (Verified Customer) (01-20-2021)

Works good for ICC in fibroblasts. High nuclear signal. Cells fixed with 4% PFA 15 min. TDP43 ab dilution 1:200 in PBST O/N 4ºC. Donkey anti-rabbit Alexafluor 568 1:500 for 1h RT.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: fibroblast cell
FH

MEIMEI (Verified Customer) (11-12-2020)

the antibody work prefect for my staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: iPSCs
FH

Stephane (Verified Customer) (10-31-2020)

Good product for staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Neuron
TDP-43 Antibody Immunofluorescence validation (1:100 dilution) in Neuron (Cat no:10782-2-AP)
FH

Marina (Verified Customer) (10-25-2020)

TDP43 in human primary fibroblasts. Loading: 10 ug in LB with DTT. TGX 4-15% gel. Blocking: 1h 3% BSA in PBST (0.1% tween). Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST (0.1% Tween) Detection: Goat anti-rabbit HRP + ECL+.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Primary Fibroblasts
TDP-43 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:10782-2-AP)
FH

Uxoa (Verified Customer) (02-18-2020)

Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human fibroblasts
TDP-43 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:10782-2-AP)
FH

Paul (Verified Customer) (01-15-2020)

Produces good IF images with minimal background.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: HEK293T
FH

Laura (Verified Customer) (01-10-2020)

Works very well

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
FH

Benjamin (Verified Customer) (01-07-2020)

Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:2500, 1:100
  • Cell Tissue Type: HEK293T
FH

Apoorva (Verified Customer) (12-18-2019)

It has great specificity over wide range of species.

  • Applications: Western Blot,
FH

Tilly (Verified Customer) (11-27-2019)

Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: IHC and WB: 1:1000. IF: 1:400
  • Cell Tissue Type: Mouse brain tissue
FH

Azita (Verified Customer) (10-04-2019)

I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/500-1/300-1/100- 1/50
  • Cell Tissue Type: NSC-34- Primary Hippocampal Neuron Culture (Rat P0-P1 Pups)
FH

Francisco (Verified Customer) (09-28-2019)

Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse
FH

Alinda (Verified Customer) (09-11-2019)

Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SHSY5Y
TDP-43 Antibody Western Blot, validation (1:1000 dilution) in SHSY5Y (Cat no:10782-2-AP)
FH

David (Verified Customer) (07-18-2019)

Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 (IB), 1:500 (ICC)
  • Cell Tissue Type: Neuroblastoma, iPSC-derived neurons
FH

George (Verified Customer) (07-07-2019)

Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Spinal Cord/Brain slices
FH

Elena (Verified Customer) (08-17-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK-293 cells
FH

Petra (Verified Customer) (03-06-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissue