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  • KD/KO Validated

TLE3 Polyclonal antibody

TLE3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11372-1-AP

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11372-1-AP targets TLE3 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE3 fusion protein Ag1930
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated Molecular Weight 772 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC015729
Gene Symbol TLE3
Gene ID (NCBI) 7090
RRIDAB_2203743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

Protocols

Product Specific Protocols
WB protocol for TLE3 antibody 11372-1-APDownload protocol
IHC protocol for TLE3 antibody 11372-1-APDownload protocol
IF protocol for TLE3 antibody 11372-1-APDownload protocol
IP protocol for TLE3 antibody 11372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP,ChIP

Nature

Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.

Authors - Abhijit Parolia
  • KD Validated
mouseWB

Cell Stem Cell

Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription.

Authors - Qi Li
mouseWB,ChIP

Cell Metab

TLE3 is a dual-function transcriptional coregulator of adipogenesis.

Authors - Villanueva Claudio J CJ
mouseWB

Cell Metab

Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.

Authors - Villanueva Claudio J CJ
  • KO Validated
humanWB,chIP

J Clin Invest

Small molecule JQ1 promotes prostate cancer invasion via BET-independent inactivation of FOXA1.

Authors - Leiming Wang
mousechIP

J Exp Med

Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity.

Authors - Shaojun Xing
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-05-2020)

I did western blots using mouse heart samples. I observed a TLE3 band with correct molecular weight of about 80kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue