UPK3A Polyclonal antibody

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  WB analysis using 55229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55229-1-AP (UPK3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse bladder using 55229-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 55229-1-AP (Uroplakin III antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UPK3A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55229-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Uroplakin III, uroplakin 3A, UPK3, UPIIIA, UPIII

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: mouse bladder tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55229-1-AP targets UPK3A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: uroplakin 3A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31-33 kDa
• GenBank Accession Number: NM_006953
• Gene Symbol: UPK3A
• Gene ID (NCBI): 7380
• RRID: AB_3670244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75631
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites.