CoraLite® Plus 488-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A7G3

Cat no : CL488-60271

Synonyms

CD20, MS4A1



Tested Applications

Positive IF detected inhuman tonsillitis tissue, human lung cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60271 targets CD20 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated Molecular Weight 297 aa, 33 kDa
GenBank Accession NumberBC002807
Gene Symbol MS4A1
Gene ID (NCBI) 931
RRIDAB_2883132
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells. This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD20 antibody CL488-60271Download protocol
Standard Protocols
Click here to view our Standard Protocols