Custom Polyclonal Antibodies

Request a Quotation Online.


Introduction

With 15 years’ experience in manufacturing quality, highly specific antibodies, Proteintech can provide you with a bespoke project that fits your individual needs. The Proteintech team invests time and effort in your project: from when we advise on the project design until the final bleed is delivered and we help with optimization. 

Download everything you need to know about custom production as a pdf here.

Reasons To Choose Proteintech® Custom Services

-  Track the status of your custom project using our online tool.

-  ISO 9001 and ISO 13485 accredited.

-  Range of modifications and supplementary services available.

-  Receive extra test bleeds for evaluation in the lab.  

Proteintech offer custom antibodies raised in the following species:

- Mouse

- Guinea pig

- Rabbit

(contact sales for antibodies raised in other species)

102-day immunization protocol

The Proteintech lab employs an extended 102-day-long immunization protocol as standard, supplemented with booster immunizations to emulate a realistic immune response. This protocol allows time for affinity maturation in the host immune system that ultimately leads to superior antibodies. See below for the immunization process:

Day 0 Pre-immune bleed
Day 1 Primary injection
Day 28 Boost 1
Day 42 Boost 2
Day 56 Test bleed and ELISA
Day 60 Boost 3
Day 74 Production bleed
Day 78 Boost 4
Day 88 Production bleed
Day 102 Final bleed


Polyclonal antibody production


Fusion Protein Antigen-Based Service

Proteintech will receive your expression construct and its scientists will express and purify the immunogen for use in your custom project.

Catalog Number: 90186

Includes:

-  Protein Expression

-  Immunization

-  Collection

-  Affinity Purification


Fusion Protein Antigen-Based Service and Cloning

Proteintech scientists will design and construct a plasmid for you to get your project started. Select this service if you know your target, but do not possess the necessary expression construct.

Catalog Number: 90136

Includes:

-  Cloning

-  Protein Expression

-  Immunization

-  Affinity Purification


Peptide Antigen- Based Service

If you require an antibody that will target a specific region of interest or one particular isoform, Proteintech’s scientists will help design and synthesize a peptide antigen before the rest of the custom production process. This service can also be used when no genetic material is readily available for antigen production.

Please note: The peptide synthesis is limited to 25 amino acids.

Catalog Number: 91246

Includes:

-  Peptide Design Consultation

-  Peptide Synthesis

-  Immunization

-  Affinity Purification


Phospho-Specific Service

Similar to the peptide antigen-based service above, but for an end product that will detect a specific phosphorylation on your target protein. 

Catalog Number: 91246PX


Acetylation-Specific Service

Similar to the peptide antigen-based service above, but for an end product that will detect a specific acetylation on your target protein. It follows the same production procedure as the phospho-specific service, but the peptide antigen is acetylated.

Catalog Number: 91246AcX


Gene Synthesis

Catalog Number: 9005


cDNA Synthesis

Catalog Number: 9007


Serum Purification

Catalog Number: Contact sales for more information.


Additional services

Certain project specifications will need add-on services to meet your project aims, these are detailed below:


Extra Amino Acids

(Peptide antigen projects) – charged per amino acid. 

Catalog Number: 90002 


Special Amino Acids 

(E.g., hyproxyproline) – Same catalog number as the extra amino acid service but will require you to state any non-proteinogenic/ non-coded aminoacids you wish to include in your peptide antigen before receiving a final quote.

Catalog Number: 90002 


Extra Phosphorylation Sites

(n = number of total phosphorylation sites, e.g., a total of two phosphorylation sites = 91246-2PX). Extra acetylation sites are charged and handled in the same manner (the catalog # in this case will be 91246nAcX).

Catalog Number: 91246nPX

Click here for  Custom Production FAQS 

Publications

Antibody:  β5 integrin
Subject: Circulating angiopoietin-like 4 links proteinuria with hypertriglyceridemia in nephrotic syndrome.

Ref:

24317117

Journal:

Nat Med

Publication date:

2013

Institution:

University of Alabama at Birmingham

Author:

Lionel C Clement

Notes:

N/A

 

Antibody: FAM8A1
Subject: Defining human ERAD networks through an integrative mapping strategy.

Ref: 

22119785

Journal:

Nat Cell Biol

Publication date:

2012

Institution:

Stanford University

Author: 

Christianson John C JC

Notes:

N/A

 

Antibody: FGF9
Subject: Cytoskeletal polarity mediates localized induction of the heart progenitor lineage.

Ref:

21785423

Journal:

Nat Cell Biol

Publication date:

2011

Institution:

University of Arizona

Author: 

Cooley James J

Notes:

Species: Ciona

Request a quotation:

Target name, Gene ID, sequence etc.










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