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Overview, pathway and related antibodies
Nuclear factor kappa B (NF-κB) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is composed of multiple subunits: RelA (p65), RelB, c-Rel, NF-κB1 (p50) and NF-κB2 (p52) (1).
These proteins function as dimeric transcription factors that regulate the expression of genes influencing a broad range of biological processes including innate and adaptive immunity, inflammation, stress responses, B-cell development, and lymphoid organogenesis (2). NF-κB exists freely in the cytoplasm, where it is inhibited by IκB proteins. In the classical (canonical) pathway, NF-κB activation occurs through the phosphorylation, ubiquitination and subsequent proteosomal degradation of IκB. An alternative (non-cononical) activation involves phosphorylation and processing of the p52 precursor, p100 into the mature protein and subsequent nuclear translocation of the RelB/p52, heterodimer that translocate to the nucleus and induce target gene expression (3).
Abnormalities in NF-κB pathway has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory (4,5).
|NF-ΚB||14220-1-AP||Rabbit Poly||ELISA, IF, IHC, IP, WB||24 Publications|
|P65; RELA - KD/KO Validated||10745-1-AP||Rabbit Poly||ELISA, FC, IF, IHC, IP, WB||128 Publications|
|NFKB2||10409-2-AP||Rabbit Poly||ELISA, IF, IHC, IP, WB||3 Publications|
|NFKB1||23576-1-AP||Rabbit Poly||ELISA, WB, IHC, IF|
|Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 14220-1-AP (NF-κB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)|