HL-60 cells were subjected to SDS PAGE followed by western blot with 13655-1-AP (IL3RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13655-1-AP (IL3RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13655-1-AP (IL3RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD45RO and 5 ul CoraLite® Plus 488 Anti-Human CD123 (CL488-65256, Clone:HI12H7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with PE Anti-Human CD45RO and 5 ul CoraLite® Plus 647 Anti-Human CD123 (CL647-65256, Clone:HI12H7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with PE Anti-Human CD45RO and 5 ul CoraLite® Plus 750 Anti-Human CD123 (CL750-65256, Clone:HI12H7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Il3ra antibody (30012-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD45RO and 5 ul PE Anti-Human CD123 (PE-65256, Clone:HI12H7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.