Lysates of untreated and IFN gamma treated A549 cells were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Untreated and PNGase F-treated lysates of human placenta were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-PD-L1/CD274 (C-terminal)(IP:28076-1-AP, 4ug; Detection:28076-1-AP 1:500) with human placenta tissue lysate 800 ug.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-L1/CD274 (C-terminal) antibody (28076-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1, red).