Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human TGF Beta 1 (21898-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IP result of anti-TGF Beta 1 (IP:21898-1-AP, 4ug; Detection:21898-1-AP 1:6000) with mouse skin tissue lysate 640 ug.

Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL.
 

Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NeutraKine® TGF beta 1 (69012-1-Ig, Clone:1E3E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.

1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TGF beta 1 (CL488-69012, Clone:1E3E9) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®647 Anti-Human TGF beta 1 (CL647-69012, Clone:1E3E9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).