IF result of MBP tag antibody (66003-1-Ig, 1:1,000) with MBP-Tagged protein.Courtesy of Neeraj Tiwari, PhD, Yale School of Medicine, Yale University.

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Recombinant protein were subjected to SDS PAGE followed by western blot with 66003-1-Ig (MBP tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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IP Result of anti-MBP tag (IP:66003-1-Ig, 5ug; Detection:66003-1-Ig 1:20000) with Recombinant protein protein lysate 800ug.

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lane1: MBP-NOL6 64kd; lane2 MBP 42kd were subjected to SDS PAGE followed by western blot with 15089-1-AP (MBP-Tag Antibody) at dilution of 1:3000.

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Various lysates were subjected to SDS PAGE followed by western blot with CL750-66003 (MBP tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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IF result of MBP tag antibody (66003-1-Ig, 1:1,000) with MBP-Tagged protein.Courtesy of Neeraj Tiwari, PhD, Yale School of Medicine, Yale University. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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Recombinant protein were subjected to SDS PAGE followed by western blot with 66003-1-Ig (MBP tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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IP result of anti-MBP tag (IP:66003-1-Ig, 5ug; Detection:66003-1-Ig 1:20000) with Recombinant protein protein lysate 800ug. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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Structure of MBP.

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MBP-Tag fusion protein Ag0942 SDS-PAGE

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Immunoprecipitation of maltose binding protein (MBP) from E. coli cell extract. The Western blot shows the very high effectivity of the MBP-Trap: No MBP is left in Flow-Through lane.I: Input, FT: Flow-Through, B: Bound.

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Immunoprecipitation of maltose binding protein (MBP) from E. coli cell extract. The Western blot shows the very high effectivity of the MBP-Trap: No MBP is left in Flow-Through lane.I: Input, FT: Flow-Through, B: Bound.

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