A431 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

mouse colon tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

rat thymus tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (60283-2-Ig, Clone: 6C4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Non-treated and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

Non-treated and Etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

Immunofluorescent analysis of (-20°C Ethanol) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (67826-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 HT-29 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (67826-1-Ig, Clone:1H6G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Non-treated and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.

Non-treated and Etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.

Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (67826-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.

1X10^6 HT-29 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (67826-1-Ig, Clone:1H6G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WB result of P53 antibody (10442-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10442-1-AP (P53 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

IP result of anti-P53 (IP:10442-1-AP, 4ug; Detection:10442-1-AP 1:1000) with A431 cells lysate 3000ug.

Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (10442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21891-1-AP (TP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of p53 antibody (21891-1-AP, 1:1000) with sh-control and sh-p53 transfected A431 cells.

A431 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP (TP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP Result of anti-TP53 (IP:21891-1-AP, 3ug; Detection:21891-1-AP 1:1000) with A431 cells lysate 1650ug.

Immunofluorescent analysis of HepG2 cells using 21891-1-AP (TP53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Non-treated HT-29 and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53(Ser15) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunofluorescent analysis of (4% PFA) fixed non-treated HT-29 and etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Non-treated HT-29, phosphatase inhibitor treated and λ phosphatase treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28960-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28959-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.

Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).