Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66220 (PD-1/CD279 antibody), at dilution of 1:150.

1X10^6 Jurkat cells untreated (blue, dashed line) or treated with CD3 antibody (OKT3, 2 ug/ml) were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human PD-1/CD279 (CL488-66220, Clone: 4H4D1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue, solid line). Cells were not fixed.

Purity of Recombinant Human PD-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Immobilized Human PD-1 (His tag) at 2 μg/mL (100 μL/well) can bind Human PD-L1 (hFc tag) with a linear range of 11-43 ng/mL.

Various lysates were subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 antibody (66220-1-Ig, Clone: 4H4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 unstimulated (dashed line) or PMA and ionomycin treated (red) MOLT-4 cells were surface stained with 0.2 ug Anti-Human PD-1/CD279 (66220-1-Ig, Clone: 4H4D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.2 ug isotype control antibody (blue, solid line). Cells were not fixed.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

mouse brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 PHA-stimulated (5 μg/mL, overnight) human peripheral blood mononuclear cells (PBMCs) were surface stained with 0.20 ug CoraLite647-conjugated Anti-Human PD-1/CD279 (CL647-66220, clone: 4H4D1) and 0.20 ug FITC-Anti-Human CD3 (FITC-65151, clone UCHT1). Cells were not fixed.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®647 PD-1/CD279 antibody (CL647-66220, Clone: 4H4D1 ) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®647 PD-1/CD279 antibody (CL647-66220, Clone: 4H4D1 ) at dilution of 1:100.

1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with 0.20 ug CoraLite647-conjugated Anti-Human PD-1/CD279 (CL647-66220, clone: 4H4D1) and 0.20 ug FITC-Anti-Human CD3 (FITC-65151, clone UCHT1). Cells were not fixed.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®647 PD-1/CD279 antibody (CL647-66220, Clone: 4H4D1 ) at dilution of 1:200, CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10, red), CoraLite®488 CD8 antibody (CL488-66868, Clone: 1G2B10, green).

Standard curve of Human PD1 ELISA Kit KE00075.

Standard curve of MP50043-1, a-Synuclein Monoclonal Matched Antibody Pair - BSA and Azide free, measured by sandwich ELISA. Capture antibody: 60285-1-PBS. Detection antibody: 66412-1-PBS (HRP conjugated). Standard: Ag1285. Range: 0.313-20 ng/mL.