Non-treated SH-SY5Y and PDGF (HZ-1215) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28866-1-AP (Phospho-Tau (Thr181) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 81383-1-RR (Phospho-TAU (Ser404) antibody) at dilution of 1:16000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 81383-1-RR (Phospho-TAU (Ser404) antibody) at dilution of 1:16000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 81383-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 40x lens).

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP (TAU antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

WB result of TAU antibody (66499-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAU transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 TAU antibody (CL488-10274) at dilution of 1:200.

MAPT fusion protein Ag35380 SDS-PAGE

Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 82568-1-RR (Phospho-TAU (Ser202/Thr205) antibody) at dilution of 1:10000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.