The time lapse analysis reveals the reorganization of actin after treatment with Cytochalasin D: HeLa cells were subjected to confocal imaging upon treatment with 2 µM of Cytochalasin D for 1 hour and recovery for 4 hours. Actin Chromobody enables monitoring of actin dynamics in real-time in living cells.
Nuclear Actin-Chromobody: NIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody (TagGFP2, green) and the Lamin-Chromobody (TagRFP, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum. Courtesy of Matthias Plessner.
HeLa cells were transfected with the Spot-Tag®-Actin plasmid overnight, fixed, stained with Spot-Label ATTO594 (product code eba594), and imaged using 40X objective. Spot-Tag®-Actin filaments in red, cell nuclei in blue (DAPI). Scale bar 10 µm.
