Halo-Trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. Coomassie and Western blot. Halo-tag antibody [28A8], monoclonal mouse IgG1and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

Halo-trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. I: Input, FT: Flow-Through, B: Bound

Chromatin Immunoprecipitation (ChIP) utilizing Halo-Trap Magnetic Agarose (otma) was performed on cross-linked chromatin isolated from the liver of a transgenic mouse line expressing a Halo-tagged version of clock factor REVERBα (HaloReverbα). Samples were isolated at timepoints of non-peak (ZT20) or peak (ZT8) binding of the REVERBα protein to the Bmal1 promoter region of the genome. The enriched DNA was then quantified by ddPCR utilizing primers directed at a gene desert (negative control region) or the Bmal1 promoter. Fold enrichment of each sample DNA is relative to that of the negative control region of each sample.

Structure of Halo.

Halo-Trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. Coomassie and Western blot. Halo-tag antibody [28A8], monoclonal mouse IgG1and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

Halo-trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. I: Input, FT: Flow-Through, B: Bound

Immunoprecipitation of Halo-tag protein with Halo-Trap.I: Input, FT: Flow-through, B: Bound.

Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Immunoprecipitation of Halo-tag protein with Halo-Trap.I: Input, FT: Flow-through, B: Bound.