1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated Rat IgG2a isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD45R (B220) (CL488-65139, Clone: RA3-6B2) (right). Cells were not fixed.
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated CD45R (B220) antibody (CL488-65139, Clone: RA3-6B2, green) at dilution of 1:400, and CoraLite® Plus 647-conjugated CD3 antibody (CL647-65077, Clone: 17A2, red).
