Untreated and PNGase F-treated lysates of HT-29 cells and Caco-2 cells were subjected to SDS PAGE followed by western blot with 66666-1-Ig (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.
1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human CD133 (66666-1-Ig, Clone:2B8A2) (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.