Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human CD24 (67627-1-Ig, Clone:1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD24 (65121-1-Ig, Clone: M1/69) and FITC anti-rat IgG2b Antibody at dilution 1:200 (green), or stained with 0.5 ug isotype control and FITC anti-rat IgG2b Antibody at dilution 1:200 (black). Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.06 ug APC-Rat IgG2b isotype control (left) or 0.06 ug APC Anti-Mouse CD24 (APC-65121, Clone: M1/69) (right). Cells were not fixed.

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Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.

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1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human CD24 (67627-1-Ig, Clone:1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.

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1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD24 (CL488-65121, Clone: M1/69) (right). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD24 (CL647-65121, Clone: M1/69). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated rat IgG2b isotype control antibody. Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse CD24 (CL750-65121, Clone:M1/69) (red), or 0.5 ug Isotype Control. Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Mouse CD24 (CL594-65121, Clone:M1/69) (red), or 0.5 ug Isotype Control. Cells were not fixed.

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