WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.
MDA-MB-231 (CD44+), HeLa (CD44+), A549 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.
1X10^6 HeLa cells were surface stained with 0.4 ug Anti-Human CD44 (15675-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.
