WB result of EPCAM antibody (21050-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

A431 cells were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 HT-29 cells were surface stained with 0.5 ug Anti-Human EPCAM/CD326 (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were not fixed.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 MCF-7 cells were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human EPCAM (CL488-21050) (red), or 0.4 ug Control Antibody. Cells were not fixed.

1X10^6 MCF-7 cells were surface stained with 0.4 ug CoraLite®555 Anti-Human EPCAM (CL555-21050) (red), or 0.4 ug Control Antibody. Cells were not fixed.