human placenta tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Untreated and IFN gamma treated A375 cells and A549 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66248-1-Ig(PD-L1/CD274 antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 MDA-MB-231 cells were stained with 0.2 ug Anti-Human PD-L1/CD274 (66248-1-Ig, Clone: 2B11D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug mosue IgG1 isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

human placenta tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

Untreated and IFN gamma treated A375 cells and A549 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66248-1-Ig(PD-L1/CD274 antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

1X10^6 MDA-MB-231 cells were stained with 0.2 ug Anti-Human PD-L1/CD274 (66248-1-Ig, Clone: 2B11D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug mosue IgG1 isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul APC Anti-Human PD-L1 (B7-H1) (APC-65081, Clone: 29E.2A3) (blue) or isotype control antibody (black). Cells were not fixed.

1X10^6 mouse splenocytes cells were stained with 0.20ug CD274 (PD-L1, B7-H1) antibody (PE-65073, red) and control antibody (blue). Cells were not fixed.

1X10^6 unstimulated or anti-CD3/CD28 treated (2 days) BALB/C mouse splenocytes were surface stained with FITC Plus Anti-Mouse CD3 (17A2) and 0.2 ug APC Anti-Mouse CD274 (PD-L1, B7-H1) (APC-65073, Clone: 10F.9G2). Cells were not fixed.

1X10^6 PHA treated (3d) human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG2b Isotype Control (blue). 1X10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated.

1X10^6 PHA treated (3d) human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (right), or Mouse IgG2b Isotype Control (left). 1X10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (center). Cells were not fixed. Lymphocytes were gated.

1x10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD274 (PD-L1, B7-H1) (65073-1-Ig, Clone:10F.9G2) and FITC-anti Rat IgG2b at dilution 1:100 and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

1X10^6 day 3 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human PD-L1 (B7-H1) (CL488-65081, Clone: 29E.2A3) (red) or Mouse IgG2b Isotype Control (blue). Unactivated PBMCs were surface stained with CL488-65081 (black). Cells were not fixed. Lymphocytes were gated.

1X10^6 day 3 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human PD-L1 (B7-H1) (CL488-65081, Clone: 29E.2A3) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.