1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1). Cells were not fixed.
1X10^6 untreated (dashed line) or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.
1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.5 ug PE Anti-Mouse CD8a (PE-65069, Clone:53-6.7) or 0.5 ug Control Antibody. Cells were not fixed.
Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 10 ul PE Anti-Human CD206 (PE-65155, Clone:15-2) (red) or PE-Mouse IgG1 isotype control (black). Cells were not fixed.
1X10^6 human whole blood cells were surface stained with 5 ul PE Anti-Human STRO-1 (PE-65184, Clone:STRO-1). Cells were not fixed. CD45 dim, CD34 positive lymphocytes were gated.
1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.2 ug PE Anti-Mouse CD4 (PE-65104, Clone:GK1.5) or 0.2 ug Isotype Control. Cells were not fixed.
1X10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug PE Anti-Mouse CD80 (B7-1) (PE-65076, Clone:16-10A1) (red), or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD3 (PE-65133, Clone:OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD3 (PE-65133, Clone: OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.
100 ul human peripheral blood were surface stained with 20.00 ul PE Anti-Human CD34 (PE-65183, Clone: QBEnd-10) and 5 ul APC Anti-Human CD45 (APC-65109, Clone: HI30), and then treated with red blood cell lysis buffer. Cells were not fixed.