WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

HeLa nuclear fraction and cytoplasmic fraction were subjected to SDS PAGE saparately, followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped, reblotted with lamin B1 (12987-1-AP) & GAPDH (60004-1-Ig) antibodies as the loading control.

IP result of anti-NF-κB p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were stained with 0.2ug p65; RELA antibody (10745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

IP result of anti-IkB Alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:15000) with HeLa cells lysate 1320 ug.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IkB Alpha (10268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IP result of anti-NFKB1,p105,p50 (IP:14220-1-AP, 4ug; Detection:14220-1-AP 1:3000) with Jurkat cells lysate 2280 ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NFKB1 antibody (14220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (14220-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH.

Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12970-1-AP (OPRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-OPRL1 (IP:12970-1-AP, 4ug; Detection:12970-1-AP 1:500) with SH-SY5Y cells lysate 2000ug.

1X10^6 SH-SY5Y cells were stained with 0.2ug OPRL1 antibody (12970-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFKB1,p105,p50 antibody (66992-1-Ig, Clone: 2G1E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (66992-1-Ig, Clone:2G1E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 human PBMCs were surface stained with 0.2 ug Atlantic Blue™ Anti-Mouse IgG1 Isotype Control (AB-66360-1, Clone:1F8D3) (red), Atlantic Blue™ Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.5 ug Cardinal Red™ Anti-Mouse IgG1 isotype control (CR-66360-1, Clone:1F8D3) (red), Cardinal Red™ Anti-Human CD45RA (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.