Various lysates were subjected to SDS PAGE followed by western blot with 13883-1-AP (PKC Iota antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-PKC Iota (IP:13883-1-AP, 4ug; Detection:13883-1-AP 1:2000) with HeLa cells lysate 1720 ug.

WB result of PKC Iota antibody (13883-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected MCF-7 cells.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Iota antibody (13883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PKC Iota (13883-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66493 (PKC iota antibody) at dilution of 1:50.

1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PKC Iota (CL488-66493, Clone:3A1B6) (red), or 0.8 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PKC Iota (CL488-13883) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PKC Iota (CL647-66493, Clone:3A1B6) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human PKC Iota (CL647-13883) (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66493 (PKC iota antibody) at dilution of 1:50.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human PKC Iota (CL594-66493, Clone:3A1B6) (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.