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C17orf81 Polyclonal antibody

C17orf81 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10162-1-AP

Synonyms

C17orf81, DERP6, ELP5, HSPC002, MST071, MSTP071, S phase 2 protein, S-phase 2 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C17orf81 Polyclonal antibody (10162-1-AP)

    WB analysis using 10162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10162-1-AP (C17orf81 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using C17orf81 Polyclonal antibody (10162-1-AP)

    WB analysis of HEK-293 using 10162-1-AP

    WB result of C17orf81 antibody (10162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C17orf81 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse brain using 10162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse brain using 10162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse brain using 10162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse brain using 10162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse heart using 10162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse heart using 10162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA375 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse brain tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10162-1-AP targets C17orf81 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C17orf81 fusion protein Ag0194
Full Name chromosome 17 open reading frame 81
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 37 and 45 kDa
GenBank Accession NumberBC002762
Gene Symbol C17orf81
Gene ID (NCBI) 23587
RRIDAB_2274975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELP5, also named as C17orf81, DERP6 and S-phase 2 protein, acts as subunit of the RNA polymerase II elongator complex. It's involved in TP53-mediated transcriptional regulation. ELP5 has some isoforms with MW 20 kDa, 30 kDa and 34-37 kDa.

Protocols

Product Specific Protocols
WB protocol for C17orf81 antibody 10162-1-APDownload protocol
IHC protocol for C17orf81 antibody 10162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols