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RNF216 Polyclonal antibody

RNF216 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10185-1-AP

Synonyms

ring finger protein 216, RNF216, TRIAD3, U7I1, UBCE7IP1, ZIN

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of U2OS cells using RNF216 Polyclonal antibody (10185-1-AP)

    WB analysis of U2OS using 10185-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW480 cells using RNF216 Polyclonal antibody (10185-1-AP)

    WB analysis of SW480 using 10185-1-AP

    SW480 cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RNF216 Polyclonal antibody (10185-1-AP)

    WB analysis using 10185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse heart tissue, U2OS cells, SW480 cells, mouse kidney tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10185-1-AP targets RNF216 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF216 fusion protein Ag0238
Full Name ring finger protein 216
Calculated Molecular Weight 48 kDa
Observed Molecular Weight57 kDa, 90-99 kDa
GenBank Accession NumberBC000787
Gene Symbol RNF216
Gene ID (NCBI) 54476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for RNF216 antibody 10185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.

Authors - Jian Zhang
View Article