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FMNL1 Polyclonal antibody

FMNL1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10395-1-AP

Synonyms

C17orf1, C17orf1B, CLL associated antigen KW 13, FHOD4, FMNL, FMNL1, formin like 1, Formin like protein 1, KW 13, Leukocyte formin

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using FMNL1 Polyclonal antibody (10395-1-AP)

    WB analysis of Raji using 10395-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FMNL1 Polyclonal antibody (10395-1-AP)

    WB analysis using 10395-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FMNL1 Polyclonal antibody (10395-1-AP)

    WB analysis of HeLa using 10395-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using FMNL1 Polyclonal antibody (10395-1-AP)

    IHC staining of human small intestine using 10395-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10395-1-AP (FMNL1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FMNL1 Polyclonal antibody (10395-1-AP)

    IF Staining of U2OS using 10395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (10395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using FMNL1 Polyclonal antibody (10395-1-AP)

    IF Staining of Saos-2 using 10395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using FMNL1 antibody (10395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inRaji cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, Saos-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:500
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10395-1-AP targets FMNL1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMNL1 fusion protein Ag0594
Full Name formin-like 1
Calculated Molecular Weight 160 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC001710
Gene Symbol FMNL1
Gene ID (NCBI) 752
RRIDAB_10895969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. The MW of FMNL1 is about 150-160 kDa. (PMID: 16494944 )

Protocols

Product Specific Protocols
WB protocol for FMNL1 antibody 10395-1-APDownload protocol
IHC protocol for FMNL1 antibody 10395-1-APDownload protocol
IF protocol for FMNL1 antibody 10395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols