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PCSK2 Polyclonal antibody

PCSK2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 10553-1-AP

Synonyms

KEX2 like endoprotease 2, NEC 2, NEC2, Neuroendocrine convertase 2, PC2, PCSK2, Prohormone convertase 2, Proprotein convertase 2, SPC2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using human PCSK2 Polyclonal antibody (10553-1-AP)

    WB analysis using 10553-1-AP

    mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using human PCSK2 Polyclonal antibody (10553-1-AP)

    WB analysis using 10553-1-AP

    mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10553-1-AP targets PCSK2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCSK2 fusion protein Ag0852
Full Name proprotein convertase subtilisin/kexin type 2
Calculated Molecular Weight70.5 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession NumberBC005815
Gene Symbol PCSK2
Gene ID (NCBI) 5126
RRIDAB_2299124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739)

Protocols

Product Specific Protocols
WB protocol for PCSK2 antibody 10553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.

Authors - Chikafumi Ozone
View Article
ratWB

J Cell Biol

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes

Authors - Min Li
View Article
mouseWB

Biochem Biophys Res Commun

Elongator regulates the melanocortin satiety pathway.

Authors - Joseph Walters
View Article
ratWB

Function (Oxf)

ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell

Authors - Kristen E Rohli
View Article