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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PCDHGC3 Polyclonal antibody (10857-1-AP)

    IHC staining of human breast cancer using 10857-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10857-1-AP (PCDHGC3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCDHGC3 Polyclonal antibody (10857-1-AP)

    IHC staining of mouse brain using 10857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10857-1-AP (PCDHGC3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCDHGC3 Polyclonal antibody (10857-1-AP)

    IHC staining of mouse brain using 10857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10857-1-AP (PCDHGC3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PCDHGC3 Polyclonal antibody (10857-1-AP)

    IF Staining of SH-SY5Y using 10857-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PCDHGC3 antibody (10857-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PCDHGC3 Polyclonal antibody

PCDHGC3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 10857-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

PC-43, PCDH2, PCDH-gamma-C3, Protocadherin gamma-C3, Protocadherin-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10857-1-AP targets PCDHGC3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDHGC3 fusion protein Ag1243 Predict reactive species
Full Name protocadherin gamma subfamily C, 3
Calculated Molecular Weight 150 kDa
GenBank Accession NumberBC019299
Gene Symbol PCDHGC3
Gene ID (NCBI) 5098
RRIDAB_2159713
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCDHGC3, a member of the PCDHγ cluster, has been described in some studies as a tumor suppressor that promotes the apoptosis of tumor cells, as well as suppresses the Wnt- and mTOR-signaling pathways, which negatively affects the growth of various tumors such as Wilms tumors, breast cancer, or prostate cancer.

Protocols

Product Specific Protocols
IHC protocol for PCDHGC3 antibody 10857-1-APDownload protocol
IF protocol for PCDHGC3 antibody 10857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols