Tested Applications
| Positive WB detected in | mouse thymus tissue, HL-60 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 10 publications below |
| IF | See 7 publications below |
Product Information
12432-1-AP targets MYO9B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3108 Product name: Recombinant human MYO9B protein Source: e coli.-derived, T-GST Tag: GST Domain: 1660-1983 aa of BC018108 Sequence: DKALLCSVCKMTCHKKCVHKIQSHCSYTYGRKGEPGVEPGHFGVCVDSLTSDKASVPIVLEKLLEHVEMHGLYTEGLYRKSGAANRTRELRQALQTDPAAVKLENFPIHAITGVLKQWLRELPEPLMTFAQYGDFLRAVELPEKQEQLAAIYAVLEHLPEANHNSLERLIFHLVKVALLEDVNRMSPGALAIIFAPCLLRCPDNSDPLTSMKDVLKITTCVEMLIKEQMRKYKVKMEEISQLEAAESIAFRRLSLLRQNAPWPLKLGFSSPYEGVLNKSPKTRDIQEEELEVLLEEEAAGGDEDREKEILIERIQSIKEEKEDI Predict reactive species |
| Full Name | myosin IXB |
| Calculated Molecular Weight | 2023 aa, 223 kDa |
| Observed Molecular Weight | 230-250 kDa |
| GenBank Accession Number | BC018108 |
| Gene Symbol | MYO9B |
| Gene ID (NCBI) | 4650 |
| RRID | AB_2148635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13459 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYO9B antibody 12432-1-AP | Download protocol |
| IP protocol for MYO9B antibody 12432-1-AP | Download protocol |
| WB protocol for MYO9B antibody 12432-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells
| ||
Nat Commun Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset | ||
J Thromb Haemost The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets. | ||
Cell Rep Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.
| ||
J Bone Miner Res The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.
| ||
J Immunol TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sammy (Verified Customer) (11-20-2023) | Lots of speckles, but also observed at focal adhesions where it is reported in the literature.
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