14-3-3E Antibody
Rabbit Polyclonal
Catalog number: 11648-2-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | A375 cells, HeLa cells, human brain tissue, human heart tissue |
| Positive IHC detected in | human gliomas |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of 14-3-3E antibody (11648-2-AP for IP and Detection) with A375 cell lysate.
A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP(14-3-3E Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human gliomas using 11648-2-AP(14-3-3E Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | 14-3-3E fusion protein ag2247 | Full name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide |
|
Calculated molecular weight |
255aa,29kd |
Observed molecular
weight |
29kd |
|
GenBank accession number |
BC000179 | Gene ID (NCBI) | 7531 |
| Gene symbol | YWHAE | Synonyms | 14-3-3E; FLJ45465; KCIP-1; MDCR; MDS |
